2025 VC-Backed Startups Based in Metro MIlwaukee
For our Metro Milwaukee Innovation DataTool, we defined the following companies as Milwaukee-area startups. They were founded since 2010, are headquartered in the seven-county Milwaukee CSA, and have completed at least one venture capital deal.
Click here for a list of the top investors in these Milwaukee-area startups. (Source: PitchBook Data, Inc.)
| Company | HQ Location | Primary Industry Code | Business Status | Company Financing Status | Total $ Raised |
| 3LP Strategic Investors | Milwaukee, WI | Other Commercial Products | Stealth | Venture Capital-Backed | 10.50 |
| 4th Dimension Sobriety | Milwaukee, WI | Other Services (B2C Non-Financial) | Generating Revenue | Corporation | |
| 7Rivers | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 6.60 |
| Ab E Discovery | Waterloo, WI | Consulting Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | 0.10 |
| Access HealthNet | Milwaukee, WI | Clinics/Outpatient Services | Generating Revenue | Venture Capital-Backed | 6.20 |
| Active Guardian | Oconomowoc, WI | Electronic Equipment and Instruments | Generating Revenue | Venture Capital-Backed | 2.00 |
| Advanced Ionics | New Berlin, WI | Energy Production | Startup | Venture Capital-Backed | 21.06 |
| AdventureCast | Fort Atkinson, WI | Information Services (B2C) | Generating Revenue | Accelerator/Incubator Backed | 0.05 |
| Alinea Engage | Oconomowoc, WI | Enterprise Systems (Healthcare) | Generating Revenue | Private Equity-Backed | 0.86 |
| Alithias | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.30 |
| All-In Peppers and Specialty Produce | Burlington, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.02 |
| Altin Labs | Milwaukee, WI | Electronic Equipment and Instruments | Out of Business | Formerly VC-backed | 0.10 |
| Aluvis | Wauwatosa, WI | Machinery (B2B) | Generating Revenue | Angel-Backed | 0.65 |
| Amiran Technologies | Oak Creek, WI | Environmental Services (B2B) | Out of Business | Formerly VC-backed | 0 |
| Ampersand | Waukesha, WI | Other Financial Services | Generating Revenue | Venture Capital-Backed | 0 |
| Animteam | Milwaukee, WI | Multimedia and Design Software | Generating Revenue | Accelerator/Incubator Backed | |
| Appion Solutions | Racine, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 1.00 |
| Aqua Innovations | Sharon, WI | Other Agriculture | Generating Revenue | Venture Capital-Backed | 1.63 |
| Astrean | Racine, WI | Business/Productivity Software | Generating Revenue | Corporation | |
| Augray | Brookfield, WI | Business/Productivity Software | Generating Revenue | Accelerator/Incubator Backed | 0.26 |
| Aurora Spectral Technologies | Shorewood, WI | Diagnostic Equipment | Out of Business | Formerly VC-backed | 2.05 |
| AxCel Technology | Hartland, WI | Network Management Software | Generating Revenue | Venture Capital-Backed | |
| BackTrack Video | West Bend, WI | Media and Information Services (B2B) | Out of Business | Formerly VC-backed | 0.50 |
| Ballsy | Genoa City, WI | Personal Products | Generating Revenue | Corporation | 0.01 |
| Birdie Bombs | Milwaukee, WI | Beverages | Generating Revenue | Venture Capital-Backed | 0 |
| Black Essence Candles | Racine, WI | Other Consumer Durables | Generating Revenue | Accelerator/Incubator Backed | |
| bLinkup | Milwaukee, WI | Social/Platform Software | Generating Revenue | Angel-Backed | 0.20 |
| Bomboard | Whitewater, WI | Recreational Goods | Out of Business | Formerly VC-backed | 1.98 |
| BoothCentral | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.01 |
| Bopdrop | New Berlin, WI | Entertainment Software | Generating Revenue | Venture Capital-Backed | 0.69 |
| BountyHuntr | Milwaukee, WI | Human Capital Services | Out of Business | Formerly VC-backed | 0.10 |
| Bridge to Cures | Wauwatosa, WI | Other Capital Markets/Institutions | Startup | Corporation | |
| Bright Cellars | Milwaukee, WI | Information Services (B2C) | Generating Revenue | Formerly VC-backed | 41.46 |
| Brightlife Innovations | Saint Francis, WI | Communication Software | Generating Revenue | Venture Capital-Backed | 0 |
| Build it Fab | Pewaukee, WI | Construction and Engineering | Generating Revenue | Accelerator/Incubator Backed | 0.25 |
| Cadens | Sullivan, WI | Alternative Energy Equipment | Generating Revenue | Accelerator/Incubator Backed | 0.02 |
| CarCareKiosk | Milwaukee, WI | Information Services (B2C) | Generating Revenue | Formerly VC-backed | 0.52 |
| Caring with Honor | Milwaukee, WI | Elder and Disabled Care | Startup | Formerly Angel backed | 0.21 |
| Carvd N Stone | Milwaukee, WI | Publishing | Generating Revenue | Accelerator/Incubator Backed | 0.10 |
| Cell Reprogramming & Therapeutics | Wauwatosa, WI | Drug Discovery | Startup | Accelerator/Incubator Backed | |
| Central Standard Craft Distillery | Milwaukee, WI | Beverages | Generating Revenue | Angel-Backed | 2.76 |
| Chocolate Rescue for Dogs | Germantown, WI | Food Products | Out of Business | Formerly Angel backed | 0.15 |
| Clearshot | Germantown, WI | Other Commercial Services | Generating Revenue | Venture Capital-Backed | |
| Clock Shadow Creamery | Milwaukee, WI | Food Products | Startup | Accelerator/Incubator Backed | 0.01 |
| COnovate | Wauwatosa, WI | Other Materials | Product Development | Venture Capital-Backed | 4.19 |
| ConsortiEX | Milwaukee, WI | Enterprise Systems (Healthcare) | Generating Revenue | Venture Capital-Backed | 11.58 |
| Corncob | Waukesha, WI | Other Commercial Products | Generating Revenue | Accelerator/Incubator Backed | |
| Cover5 | Milwaukee, WI | Entertainment Software | Out of Business | Formerly VC-backed | 1.46 |
| CricClubs | Milwaukee, WI | Entertainment Software | Generating Revenue | Venture Capital-Backed | 5.05 |
| CrossKudi | Wauwatosa, WI | Financial Software | Generating Revenue | Venture Capital-Backed | 0.12 |
| CrowdFounder | Milwaukee, WI | Financial Software | Out of Business | Formerly VC-backed | 0.05 |
| CrowdSling | Glendale, WI | Social Content | Out of Business | Formerly Angel backed | 0.04 |
| CyteGen | Shorewood, WI | Discovery Tools (Healthcare) | Generating Revenue | Venture Capital-Backed | 2.10 |
| Dairyvative Technologies | Markesan, WI | Beverages | Out of Business | Formerly VC-backed | 4.00 |
| Dead Bird Brewing | Milwaukee, WI | Beverages | Generating Revenue | Corporation | |
| Distribution Depot | Brookfield, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 1.28 |
| Double B’s BBQ | West Allis, WI | Restaurants and Bars | Generating Revenue | Formerly Accelerator/Incubator backed | |
| Driver’s Seat | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.32 |
| DroneAdair | Sussex, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.22 |
| Dynamis Software | Milwaukee, WI | Business/Productivity Software | Profitable | Formerly VC-backed | 3.68 |
| Ease (Business/Productivity Software) | Milwaukee, WI | Business/Productivity Software | Bankruptcy: Liquidation | Formerly VC-backed | 0.52 |
| eCourt Reporters | Burlington, WI | Legal Services (B2B) | Generating Revenue | Venture Capital-Backed | 0.27 |
| EDCS Power | Mequon, WI | Electrical Equipment | Out of Business | Formerly VC-backed | 1.45 |
| Elsie Mae’s Sweet Shop | Lake Mills, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Emergent Labs | Milwaukee, WI | Other Commercial Services | Out of Business | Corporation | |
| EmOpti | Brookfield, WI | Enterprise Systems (Healthcare) | Generating Revenue | Venture Capital-Backed | 7.29 |
| Energy Tech Innovations | Milwaukee, WI | Energy Production | Generating Revenue | Accelerator/Incubator Backed | 0.05 |
| Estrigenix Therapeutics | Milwaukee, WI | Drug Discovery | Generating Revenue | Angel-Backed | 0.15 |
| Etyon | Milwaukee, WI | Enterprise Systems (Healthcare) | Generating Revenue | Private Equity-Backed | |
| EVEN (Information Services) | Milwaukee, WI | Media and Information Services (B2B) | Generating Revenue | Venture Capital-Backed | 4.25 |
| EVERSANA | Milwaukee, WI | Practice Management (Healthcare) | Generating Revenue | Private Equity-Backed | 1405.05 |
| Evolve Brands | Jackson, WI | Food Products | Out of Business | Formerly PE-Backed | 0.87 |
| Exit 7C | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 1.12 |
| FaB Wisconsin | Milwaukee, WI | Food Products | Startup | Corporation | |
| Field Better | Milwaukee, WI | Food Products | Generating Revenue | Venture Capital-Backed | 4.03 |
| Field to Freezer | Hartland, WI | Media and Information Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | |
| First Patient | Milwaukee, WI | Other Healthcare Services | Generating Revenue | Formerly Angel backed | 0.53 |
| Fiveable | Milwaukee, WI | Educational and Training Services (B2C) | Generating Revenue | Venture Capital-Backed | 13.76 |
| Flamingo Electric | Grafton, WI | Marine | Generating Revenue | Venture Capital-Backed | 11.55 |
| Float Milwaukee | Milwaukee, WI | Leisure Facilities | Generating Revenue | Formerly Angel backed | 0.01 |
| Flying Spider | Milwaukee, WI | Other Commercial Products | Stealth | Venture Capital-Backed | 1.05 |
| Forj | Waukesha, WI | Business/Productivity Software | Generating Revenue | Formerly VC-backed | 22.00 |
| Forsake | Glendale, WI | Footwear | Generating Revenue | Formerly VC-backed | 1.37 |
| FreeStyle Peppers | Mount Pleasant, WI | Food Products | Out of Business | Formerly Accelerator/Incubator backed | 0.02 |
| Frontdesk | Milwaukee, WI | Real Estate Services (B2C) | Out of Business | Formerly VC-backed | 28.70 |
| Funky Fresh Spring Rolls | Milwaukee, WI | Food Products | Generating Revenue | Corporate Backed or Acquired | 0.01 |
| FuzzPop Workshop | Milwaukee, WI | Movies, Music and Entertainment | Generating Revenue | Accelerator/Incubator Backed | 0.08 |
| Gathering Place Brewing Company | Milwaukee, WI | Other Restaurants, Hotels and Leisure | Generating Revenue | Corporation | |
| Gearbox Express | Mukwonago, WI | Industrial Supplies and Parts | Generating Revenue | Formerly VC-backed | 6.30 |
| Genlines | Oconomowoc, WI | Social/Platform Software | Startup | Angel-Backed | 0.01 |
| Geno.Me | Milwaukee, WI | Medical Records Systems | Generating Revenue | Venture Capital-Backed | 2.80 |
| GenoPalate | Milwaukee, WI | Information Services (B2C) | Generating Revenue | Venture Capital-Backed | 13.07 |
| Getter ( Business/Productivity Software) | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly Angel backed | 0.05 |
| Good City Brewing | Milwaukee, WI | Beverages | Generating Revenue | Formerly VC-backed | 0.52 |
| Gorilly Goods | Jackson, WI | Food Products | Generating Revenue | Formerly Accelerator/Incubator backed | 0.00 |
| Gostrive | Fort Atkinson, WI | Social/Platform Software | Out of Business | Formerly VC-backed | 0.68 |
| Greater States Sports Authority | Brookfield, WI | Educational and Training Services (B2C) | Generating Revenue | Formerly Angel backed | 0.28 |
| GYBITG | Milwaukee, WI | Educational and Training Services (B2C) | Startup | Accelerator/Incubator Backed | 0.01 |
| HaloVino | WhitefishBay, WI | Other Consumer Durables | Generating Revenue | Venture Capital-Backed | |
| Handdii | Grafton, WI | Financial Software | Generating Revenue | Venture Capital-Backed | 10.80 |
| hankr | Milwaukee, WI | Information Services (B2C) | Generating Revenue | Corporation | |
| HC Intellect | Milwaukee, WI | IT Consulting and Outsourcing | Generating Revenue | Venture Capital-Backed | 1.20 |
| HDL Diagnostics | Fox Point, WI | Pharmaceuticals | Out of Business | Formerly VC-backed | |
| Healthfuse | Milwaukee, WI | Enterprise Systems (Healthcare) | Generating Revenue | Private Equity-Backed | |
| HealthLX | Grafton, WI | Enterprise Systems (Healthcare) | Generating Revenue | Venture Capital-Backed | 3.41 |
| Hitlist | Milwaukee, WI | Media and Information Services (B2B) | Out of Business | Formerly VC-backed | 0.07 |
| Hjarta Care | Waukesha, WI | Surgical Devices | Startup | Venture Capital-Backed | 0.85 |
| Hopscot.ch | Milwaukee, WI | Communication Software | Out of Business | Formerly Angel backed | 0.05 |
| Houstr | Racine, WI | Specialty Retail | Generating Revenue | Venture Capital-Backed | 0.30 |
| HSM | Milwaukee, WI | Other Consumer Durables | Out of Business | Formerly VC-backed | 0.20 |
| Hyde Expedition | Milwaukee, WI | Recreational Goods | Generating Revenue | Corporation | |
| Hydrotech Innovations | Mequon, WI | Business/Productivity Software | Startup | Corporation | |
| iDAvatars | Mequon, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 6.01 |
| Ideawake | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 4.16 |
| Imagen Energy | Wauwatosa, WI | Electrical Equipment | Startup | Accelerator/Incubator Backed | |
| iMoney | Waukesha, WI | Financial Software | Startup | Angel-Backed | |
| Informed360 | Shorewood, WI | Business/Productivity Software | Generating Revenue | Formerly Accelerator/Incubator backed | 0.18 |
| Ingantec | Milwaukee, WI | Electrical Equipment | Generating Revenue | Venture Capital-Backed | 2.15 |
| Insuraviews | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | |
| Intelligent Composites | Milwaukee, WI | Road | Generating Revenue | Corporation | |
| InVekTek | Waukesha, WI | Electronic Equipment and Instruments | Generating Revenue | Venture Capital-Backed | |
| Iron Infidel | Brookfield, WI | Other Consumer Durables | Generating Revenue | Venture Capital-Backed | |
| Jackson’s Honest | Muskego, WI | Food Products | Generating Revenue | Venture Capital-Backed | 4.00 |
| JOYhelps | Elkhorn, WI | Educational and Training Services (B2C) | Generating Revenue | Venture Capital-Backed | 2.20 |
| Joystik Life | Delafield, WI | Application Software | Generating Revenue | Venture Capital-Backed | 0.72 |
| Kaliber Imaging | Whitewater, WI | Monitoring Equipment | Out of Business | Formerly Accelerator/Incubator backed | |
| Kettle Range Meat | Milwaukee, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Kinsa | Saint Francis, WI | Clothing | Generating Revenue | Venture Capital-Backed | |
| KTB Disc Jockey | Milwaukee, WI | Educational and Training Services (B2C) | Out of Business | Formerly Accelerator/Incubator backed | |
| LatinoPro | Cedarburg, WI | Human Capital Services | Generating Revenue | Formerly Angel backed | 0.06 |
| LeakShark | Milwaukee, WI | Electrical Equipment | Out of Business | Formerly Accelerator/Incubator backed | 0.02 |
| Legacy Steel (Milwaukee) | Milwaukee, WI | Construction and Engineering | Generating Revenue | Venture Capital-Backed | 10.00 |
| Lifebook | Racine, WI | Application Software | Out of Business | Formerly Accelerator/Incubator backed | 0.00 |
| Lillipad | Waukesha, WI | Business Equipment and Supplies | Generating Revenue | Venture Capital-Backed | 1.20 |
| LinqThingz | Mequon, WI | Electronic Equipment and Instruments | Out of Business | Formerly VC-backed | |
| Lucendity | Milwaukee, WI | Information Services (B2C) | Out of Business | Formerly VC-backed | 0.10 |
| LumosWifi | Milwaukee, WI | IT Consulting and Outsourcing | Generating Revenue | Formerly Angel backed | |
| LW Aqua | Sharon, WI | Other Commercial Products | Out of Business | Formerly Angel backed | 0.65 |
| ManageCore | Franklin, WI | Systems and Information Management | Generating Revenue | Private Equity-Backed | 1.99 |
| Marathon (Household Appliances) | Milwaukee, WI | Household Appliances | Out of Business | Formerly VC-backed | 2.82 |
| MCT Digital | Milwaukee, WI | Machinery (B2B) | Generating Revenue | Formerly PE-Backed | 0.75 |
| MediCardia Health | Mequon, WI | Enterprise Systems (Healthcare) | Generating Revenue | Venture Capital-Backed | 4.00 |
| MedServe | Milwaukee, WI | Other Devices and Supplies | Generating Revenue | Venture Capital-Backed | 0.73 |
| MedTrak | Wauwatosa, WI | Other Healthcare Services | Out of Business | Formerly Angel backed | 0.10 |
| Meeper | Whitewater, WI | Other Hardware | Generating Revenue | Venture Capital-Backed | 0.14 |
| MeterHero (Acquired) | Milwaukee, WI | Application Software | Startup | Formerly Accelerator/Incubator backed | 0.75 |
| Midwest Express | Milwaukee, WI | Air | Generating Revenue | Venture Capital-Backed | 1.00 |
| Mikroflot | Milwaukee, WI | Environmental Services (B2B) | Generating Revenue | Formerly VC-backed | 0.05 |
| Milwaukee Pretzel Company | Milwaukee, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Milwaukee Swap | Milwaukee, WI | Footwear | Out of Business | Formerly Accelerator/Incubator backed | |
| Mindful Staffing Solutions | Milwaukee, WI | Human Capital Services | Generating Revenue | Venture Capital-Backed | 0.40 |
| MindLight | Butler, WI | Other Healthcare Technology Systems | Generating Revenue | Corporation | |
| MobCraft | Milwaukee, WI | Beverages | Generating Revenue | Accelerator/Incubator Backed | 1.12 |
| Motivo (Therapeutic Devices) | Franklin, WI | Therapeutic Devices | Generating Revenue | Venture Capital-Backed | 4.81 |
| Mpirik | Milwaukee, WI | Enterprise Systems (Healthcare) | Generating Revenue | Formerly VC-backed | 7.80 |
| Mr. Wings (Restaurants and Bars) | Milwaukee, WI | Restaurants and Bars | Generating Revenue | Accelerator/Incubator Backed | |
| MSI Data | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Private Equity-Backed | |
| MūL Technologies | Mequon, WI | Electronic Equipment and Instruments | Generating Revenue | Venture Capital-Backed | |
| Mumbies | Milwaukee, WI | Other Consumer Durables | Generating Revenue | Accelerator/Incubator Backed | |
| MuSample | Milwaukee, WI | Information Services (B2C) | Generating Revenue | Accelerator/Incubator Backed | |
| MyPhame | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 0.27 |
| MySureFit | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.81 |
| Nano Red Biotech | Wauwatosa, WI | Drug Discovery | Out of Business | Formerly Accelerator/Incubator backed | 0.25 |
| Neostella | Milwaukee, WI | IT Consulting and Outsourcing | Generating Revenue | Venture Capital-Backed | 10.70 |
| Netless Catch | Milwaukee, WI | Food Products | Out of Business | Formerly VC-backed | |
| New River Technologies | Grafton, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | |
| New Works | Milwaukee, WI | Consulting Services (B2B) | Out of Business | Formerly Accelerator/Incubator backed | |
| Nota3D | Mount Pleasant, WI | Machinery (B2B) | Generating Revenue | Accelerator/Incubator Backed | 0.30 |
| Novera/Ixonia | Ixonia, WI | Other Commercial Products | Out of Business | Formerly VC-backed | 1.80 |
| Novir | Milwaukee, WI | Clinics/Outpatient Services | Generating Revenue | Venture Capital-Backed | 3.89 |
| Okanjo | Milwaukee, WI | Media and Information Services (B2B) | Generating Revenue | Formerly VC-backed | 3.39 |
| Oncata | Milwaukee, WI | Media and Information Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | |
| OnKöl | Milwaukee, WI | Monitoring Equipment | Generating Revenue | Venture Capital-Backed | 3.00 |
| OpenPitch | New Berlin, WI | Financial Software | Generating Revenue | Venture Capital-Backed | 0.41 |
| Operose Advisors | Milwaukee, WI | Asset Management | Generating Revenue | Venture Capital-Backed | |
| Optimyl Benefits | Milwaukee, WI | Commercial/Professional Insurance | Generating Revenue | Accelerator/Incubator Backed | |
| Otto Sciences | Waukesha, WI | Media and Information Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | 0.02 |
| P4 Infrastructure | Milwaukee, WI | Electronic Equipment and Instruments | Generating Revenue | Accelerator/Incubator Backed | |
| Part Analytics | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Formerly VC-backed | 3.01 |
| Parts to Move | Brookfield, WI | Internet Retail | Out of Business | Formerly Accelerator/Incubator backed | 0.01 |
| Pear Commerce | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 18.25 |
| PegEx | Waukesha, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 31.94 |
| Penrod | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 33.00 |
| Photavia | Franklin, WI | Movies, Music and Entertainment | Generating Revenue | Angel-Backed | 0.40 |
| Pinewood Creek Apartments | New Berlin, WI | Real Estate Services (B2C) | Out of Business | Formerly VC-backed | 5.09 |
| PortfolioBay | West Allis, WI | Business/Productivity Software | Generating Revenue | Accelerator/Incubator Backed | |
| Press Premium Alcohol Seltzer | Milwaukee, WI | Beverages | Generating Revenue | Venture Capital-Backed | |
| PrintFoam | Wales, WI | Other Commercial Services | Generating Revenue | Venture Capital-Backed | 1.50 |
| PROMISS Diagnostics | Wauwatosa, WI | Diagnostic Equipment | Generating Revenue | Venture Capital-Backed | 0.40 |
| Protein Foundry | Milwaukee, WI | Biotechnology | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Proximity Malt | Milwaukee, WI | Cultivation | Generating Revenue | Private Equity-Backed | 60.00 |
| Pulsed Burst Systems | Milwaukee, WI | Other Commercial Products | Generating Revenue | Accelerator/Incubator Backed | 0.05 |
| PumpHero | Milwaukee, WI | Information Services (B2C) | Out of Business | Formerly Accelerator/Incubator backed | 0.10 |
| Puresine | Milwaukee, WI | Energy Storage | Out of Business | Formerly Accelerator/Incubator backed | 0.04 |
| Pythonic | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 0.09 |
| Radian IoT | Okauchee, WI | Electrical Equipment | Generating Revenue | Venture Capital-Backed | 6.50 |
| RadPoker | Milwaukee, WI | Entertainment Software | Out of Business | Formerly VC-backed | |
| RAIC Labs | Delafield, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 33.40 |
| Rapid Radicals Technology | Milwaukee, WI | Environmental Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | 0.10 |
| Ready Rebound | Milwaukee, WI | Other Healthcare Services | Generating Revenue | Venture Capital-Backed | 5.10 |
| RecruitChute | Whitewater, WI | Business/Productivity Software | Startup | Accelerator/Incubator Backed | |
| Remedy Analytics | Milwaukee, WI | Media and Information Services (B2B) | Generating Revenue | Formerly Angel backed | |
| Renaissant | Sussex, WI | Logistics | Generating Revenue | Venture Capital-Backed | 9.07 |
| Reneurogen | Elm Grove, WI | Drug Discovery | Generating Revenue | Accelerator/Incubator Backed | 0.03 |
| Rent College Pads | Milwaukee, WI | Other Restaurants, Hotels and Leisure | Generating Revenue | Private Equity-Backed | 14.00 |
| Retham Technologies | Wauwatosa, WI | Diagnostic Equipment | Generating Revenue | Venture Capital-Backed | 0.05 |
| Returns on Demand | Delafield, WI | Application Software | Generating Revenue | Venture Capital-Backed | 0.58 |
| Revolution Computing | Lake Mills, WI | Application Specific Semiconductors | Startup | Corporation | |
| Rex K-12 | Milwaukee, WI | Educational Software | Generating Revenue | Venture Capital-Backed | 0.21 |
| Right Track | Slinger, WI | Other Commercial Services | Stealth | Venture Capital-Backed | 0.24 |
| Rising Tides Analytics | Milwaukee, WI | Consulting Services (B2B) | Generating Revenue | Venture Capital-Backed | 0.20 |
| Rivermark Medical | Milwaukee, WI | Therapeutic Devices | Clinical Trials – Phase 2 | Venture Capital-Backed | 34.69 |
| Rivet (Business/Productivity Software) | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | |
| RoddyMedical | Wauwatosa, WI | Therapeutic Devices | Generating Revenue | Venture Capital-Backed | 2.15 |
| Royal Palm Foods | Delafield, WI | Logistics | Out of Business | Formerly Angel backed | 0.25 |
| RPRDx | Milwaukee, WI | Other Pharmaceuticals and Biotechnology | Generating Revenue | Accelerator/Incubator Backed | |
| Sabhya Technologies | Brookfield, WI | IT Consulting and Outsourcing | Generating Revenue | Formerly Accelerator/Incubator backed | 0.02 |
| SafedoorPM | Jackson, WI | Business/Productivity Software | Generating Revenue | Formerly Angel backed | 1.12 |
| Safelumin | Oconomowoc, WI | Electronics (B2C) | Generating Revenue | Venture Capital-Backed | 0.14 |
| Safepro Technologies | Whitewater, WI | Electrical Equipment | Generating Revenue | Accelerator/Incubator Backed | |
| SAFIO Solutions | Brookfield, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 3.15 |
| Scanalytics | Milwaukee, WI | Electronic Equipment and Instruments | Generating Revenue | Venture Capital-Backed | 4.12 |
| Secchi | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 2.06 |
| Secure Bridges | Milwaukee, WI | Other Services (B2C Non-Financial) | Out of Business | Formerly Accelerator/Incubator backed | 0.11 |
| Securus Data Centers | Milwaukee, WI | Systems and Information Management | Out of Business | Formerly Angel backed | 0.72 |
| Sell-On | Delafield, WI | Media and Information Services (B2B) | Out of Business | Formerly Accelerator/Incubator backed | 0.01 |
| Septillionth | Milwaukee, WI | Electronic Equipment and Instruments | Generating Revenue | Accelerator/Incubator Backed | |
| Seven Marine | Germantown, WI | Marine | Generating Revenue | Formerly VC-backed | 0.62 |
| Shake Escrow | Brookfield, WI | Other Commercial Products | Stealth | Angel-Backed | 0.01 |
| Sharla’s Coffee Stop | Milton, WI | Beverages | Generating Revenue | Accelerator/Incubator Backed | |
| Shift Savings | Delafield, WI | Financial Software | Generating Revenue | Formerly Accelerator/Incubator backed | |
| Shindigs | Milwaukee, WI | Social/Platform Software | Out of Business | Formerly Angel backed | 0.10 |
| Shockray Self Defense | Oak Creek, WI | Electrical Equipment | Out of Business | Formerly VC-backed | 0.05 |
| Sift Healthcare | Milwaukee, WI | Enterprise Systems (Healthcare) | Generating Revenue | Venture Capital-Backed | 33.79 |
| SimaFore | Wauwatosa, WI | IT Consulting and Outsourcing | Generating Revenue | Corporate Backed or Acquired | |
| Sky City Data | Milwaukee, WI | IT Consulting and Outsourcing | Startup | Angel-Backed | 0.50 |
| Socialeads | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 3.71 |
| Soul Brew Kombucha | Milwaukee, WI | Beverages | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Soul Mobility | Oconomowoc, WI | Therapeutic Devices | Generating Revenue | Venture Capital-Backed | 0.57 |
| SoulBoxer | East Troy, WI | Beverages | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| SourcingUSA | Milwaukee, WI | Media and Information Services (B2B) | Out of Business | Formerly VC-backed | |
| SpotHopper | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Venture Capital-Backed | 26.00 |
| Steady Shot | Oconomowoc, WI | Therapeutic Devices | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| SteamChain (Business/Productivity Software) | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 1.07 |
| Stewdio (YC F24) | New York, NY | Multimedia and Design Software | Generating Revenue | Accelerator/Incubator Backed | 0.50 |
| Stonehouse Water Technologies | Milwaukee, WI | Electronics (B2C) | Generating Revenue | Formerly VC-backed | 1.55 |
| StyleQ | Milwaukee, WI | Other Restaurants, Hotels and Leisure | Generating Revenue | Accelerator/Incubator Backed | |
| Subatomic AI | Menomonee Falls, WI | IT Consulting and Outsourcing | Generating Revenue | Venture Capital-Backed | 2.50 |
| Summary Medical | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly Accelerator/Incubator backed | 0.08 |
| Supernola | Jackson, WI | Food Products | Out of Business | Formerly VC-backed | 2.50 |
| Sustainable America | Milwaukee, WI | Environmental Services (B2B) | Generating Revenue | Formerly Accelerator/Incubator backed | |
| Swirl Insurance Services | Brookfield, WI | Information Services (B2C) | Out of Business | Formerly Angel backed | 1.80 |
| Sydetasker | Milwaukee, WI | Other Services (B2C Non-Financial) | Generating Revenue | Accelerator/Incubator Backed | 0.02 |
| System Seltzers | Milwaukee, WI | Beverages | Generating Revenue | Accelerator/Incubator Backed | 0.47 |
| T4 Solutions | Pewaukee, WI | Other Commercial Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| TAI Diagnostics | Wauwatosa, WI | Diagnostic Equipment | Out of Business | Formerly VC-backed | 24.00 |
| TaskCause | Milwaukee, WI | Other Services (B2C Non-Financial) | Out of Business | Formerly VC-backed | 0.20 |
| TechCanary | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Private Equity-Backed | 0.50 |
| Ten Bay Bio | Milwaukee, WI | Clinics/Outpatient Services | Stealth | Venture Capital-Backed | 11.55 |
| Tevetron | New Berlin, WI | Internet Service Providers | Generating Revenue | Venture Capital-Backed | |
| The Financial Promise | Milwaukee, WI | Educational and Training Services (B2C) | Generating Revenue | Venture Capital-Backed | |
| The Good Jobs | Milwaukee, WI | Business/Productivity Software | Out of Business | Formerly VC-backed | 0.82 |
| The Grand | Glendale, WI | Educational and Training Services (B2C) | Generating Revenue | Venture Capital-Backed | 7.07 |
| The Naked Baker | New Berlin, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| The Pink Bakery | Menomonee Falls, WI | Food Products | Generating Revenue | Venture Capital-Backed | 0.40 |
| TheMINIClassy | Milwaukee, WI | Clothing | Out of Business | Formerly Accelerator/Incubator backed | |
| Tidal Financial Group | Milwaukee, WI | Asset Management | Generating Revenue | Private Equity-Backed | 32.15 |
| Tip a ScRxipt | Milwaukee, WI | Financial Software | Generating Revenue | Venture Capital-Backed | 0.63 |
| Top Note | Milwaukee, WI | Beverages | Generating Revenue | Venture Capital-Backed | 0.05 |
| TRT Speech Labs | Racine, WI | Other Healthcare Technology Systems | Generating Revenue | Accelerator/Incubator Backed | |
| Tundra Targeted Therapeutics | Milwaukee, WI | Biotechnology | Startup | Venture Capital-Backed | 0.44 |
| TwoLips Creative | Milwaukee, WI | Media and Information Services (B2B) | Generating Revenue | Venture Capital-Backed | |
| URTPC | Milwaukee, WI | Other Hardware | Generating Revenue | Venture Capital-Backed | |
| VasoGnosis | Milwaukee, WI | Diagnostic Equipment | Out of Business | Formerly VC-backed | 1.43 |
| Venture With Impact | Milwaukee, WI | Other Commercial Services | Startup | Formerly Accelerator/Incubator backed | |
| Verdico AI | Milwaukee, WI | Business/Productivity Software | Generating Revenue/Not Profitable | Venture Capital-Backed | 6.00 |
| Vibrant Body Company | Milwaukee, WI | Clothing | Generating Revenue | Venture Capital-Backed | 0.15 |
| Vivid Microscopy | Wauwatosa, WI | Discovery Tools (Healthcare) | Generating Revenue | Corporation | |
| Vytal Health | Milwaukee, WI | Clinics/Outpatient Services | Generating Revenue | Angel-Backed | 1.40 |
| Wallit (Business/Productivity Software) | Brookfield, WI | Business/Productivity Software | Generating Revenue | Formerly VC-backed | 1.00 |
| Wantable | Milwaukee, WI | Clothing | Generating Revenue | Venture Capital-Backed | 6.59 |
| washbnb | Milwaukee, WI | Other Services (B2C Non-Financial) | Generating Revenue | Accelerator/Incubator Backed | 0.36 |
| Watton | Bayside, WI | Business/Productivity Software | Out of Business | Formerly Angel backed | 0.12 |
| WayAround | Bayside, WI | Other Services (B2C Non-Financial) | Out of Business | Formerly VC-backed | |
| Wear Tech | Hartland, WI | Electronics (B2C) | Product In Beta Test | Venture Capital-Backed | 2.66 |
| Wellntel | Milwaukee, WI | Electronic Equipment and Instruments | Generating Revenue | Venture Capital-Backed | 4.50 |
| WilCraft Can | Oak Creek, WI | Printing Services (B2B) | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| Wisconsin Soup Company | Wauwatosa, WI | Food Products | Generating Revenue | Accelerator/Incubator Backed | 0.01 |
| XLock Biosciences | West Allis, WI | Biotechnology | Generating Revenue | Corporation | |
| Zero Locus | Milwaukee, WI | Database Software | Out of Business | Formerly VC-backed | 0.50 |
| Zizzl Health | Milwaukee, WI | Human Capital Services | Generating Revenue | Venture Capital-Backed | 25.00 |
| ZoomShift | Milwaukee, WI | Business/Productivity Software | Generating Revenue | Formerly Accelerator/Incubator backed | 0.06 |